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Status |
Public on Jun 12, 2013 |
Title |
PA_36 |
Sample type |
genomic |
|
|
Source name |
Frozen pilocytic astrocytoma tumor tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: Pilocytic astrocytoma tumor brain location: Cerebellum age at diagnosis: 4 y gender: Male dna methylation subgroup: 2 mapk hit: KIAA1549:BRAF fusion_16:9
|
Treatment protocol |
Tumor samples were snap frozen upon resection and stored at -80 oC until DNA extraction. Tumor content was assessed from equivalent material fixed in formalin and used for histological diagnosis (all samples contained ≥70% tumor cells).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
High molecular weight DNA was extracted by use of a salting-out method or by ultracentrifugation over cesium chloride
|
Label |
Biotin/DNP
|
Label protocol |
standard Illumina protocol
|
|
|
Hybridization protocol |
standard Illumina protocol
|
Scan protocol |
Illumina iScan System according to the manufacturer's protocol.
|
Description |
Pilocytic astrocytoma, Cerebellum, 4, Male, 2, KIAA1549:BRAF fusion_16:9
|
Data processing |
Data analysis was performed using GenomeStudio 2011.1 (methylationmodule 1.9.0) and anootation 1.2. The methylation analysis was performed as recommended by Illumina, with no background subtraction and normalisation to internal controls.
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|
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Submission date |
Feb 26, 2013 |
Last update date |
Jun 12, 2013 |
Contact name |
Sally R Lambert |
E-mail(s) |
sally_lambert40@hotmail.com
|
Organization name |
Queen Mary, University of London
|
Department |
Centre for Cutaneous Research
|
Street address |
Blizard Institute, 4 Newark Street
|
City |
London |
ZIP/Postal code |
E1 2AT |
Country |
United Kingdom |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE44684 |
DNA methylation data from pilocytic astrocytoma tumor samples and normal cerebellum controls |
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