|
| Status |
Public on Dec 31, 2014 |
| Title |
heart/t3 |
| Sample type |
RNA |
| |
|
| Source name |
left ventrical/heart
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: heart let ventricle
disease state: diabetic cardiomyopathy
gender: male
strain: Wistar
age: adult
|
| Treatment protocol |
Diabetes in Wistar rats was induced by feeding them with high-fat diet for 4 weeks followed by two Intraperitoneal injection of streptozotocin (STZ, 30mg/kg body weight), at one week interval.Non-diabetic rats was be fed with regular chow for 4 weeks followed by two Intraperitoneal injection of citrate buffer (pH 4.4, 0.05 M at a dose of 0.25 ml/kg body weight), as vehicle, at an interval of one week. Then after both Diabetic and non-diabetic rats were fed on high fat and normal chow diet, respectively and were sacrificed at 12 weeks after the second STZ injection. Diabetes in Wistar rats was confirmed by increased fasting blood glucose level (556.25±107.68mg/dl), Total Cholesterol (139.63±55.35mg/dl) & Triglycerides (78.93±21mg/dl), at the time of sacrifice. Intraperitoneal glucose tolerance test (IPGTT) and Intraperitoneal insulin resistance test (IPIRT) confirms glucose tolerance and insulin sensitivity in Diabetic rats. DCM in Wistar rats was confirmed by the 1.6 fold increased heart to body weight ratio and presence of non-ischemic lesions, interstitial and perivascular fibrosis as well as myocytes atrophy and hypertrophy, on gross and microscopic examination of the heart.
|
| Growth protocol |
Wistar rats were housed in standard polypropylene cages (2 rats/cage) and maintained under controlled room temperature and humidity with 12/12-hour light-dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy mini kit Qiagen.
|
| Label |
biotin
|
| Label protocol |
FlashTag RNA Labeling with Genisphere RNA Labeling Kit
|
| |
|
| Hybridization protocol |
Following labeling, 21.5 ul of biotin-labeled RNA was hybridized for 16 hr at 48C on Affymetrix miRNA 2.0 Arrays. The miRNA 2.0 Arrays were washed and stained in the Affymetrix Fluidics Station 450 using fludics script FS450_0003.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 30007G..
|
| Data processing |
Data files were analyzed with Affymetrix miRNAQCtool using RMA background correction and quantile normalization (Default settings). Further normalized data also analysed follwed by differentiall miRNA expression and cluster analysis using GeneSpring GX12.0 sofware.
|
| |
|
| Submission date |
Feb 08, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
madhu khullar |
| E-mail(s) |
akhilesh004@gmail.com
|
| Phone |
0172-2755229
|
| Organization name |
Postgraduate Institute of Medical Education and Research
|
| Department |
Experimental Medicine and Biotechnology
|
| Street address |
Research Block-B
|
| City |
Chandigarh |
| State/province |
Chandigarh |
| ZIP/Postal code |
160012 |
| Country |
India |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE44179 |
Role of micro-RNAs in pathophysiology of diabetic cardiomyopathy. |
|
