|
| Status |
Public on Sep 30, 2007 |
| Title |
E2AB |
| Sample type |
RNA |
| |
|
| Source name |
mesenteric arteries
|
| Organism |
Rattus norvegicus |
| Characteristics |
Female ovariectomized Sprague-Dawley rat, 5.5 weeks of age treated for 4 days with estradiol benzoate (0.15 mg/kg/day)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mesenteric vasculature was dissected free from the gastrointestinal tract and placed in RNAlater (Ambion). The mesenteric arteries were dissected free from the surrounding fat and connective tissues while immersed in RNAlater (Rodrigo et al., J Pharm Tox Meth 47(2):87-92, 2002). The mesenteric arteries were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated by spectrophotometry and agarose gel electrophoresis.
|
| Label |
Alexa-647
|
| Label protocol |
see CodeLink application notes and manuals
|
| |
|
| Hybridization protocol |
see CodeLink application notes and manuals
|
| Scan protocol |
CodeLink Bioarrays scanned on Agilent G2565 microarray scanner
|
| Description |
Samples E2AA and E2AB are technical replicates derived from the same cRNA. Mesenteric arteries were dissected free from surrounding fat, connective tissue and veins while submerged under RNAlater.
|
| Data processing |
global median normalization
|
| |
|
| Submission date |
May 02, 2006 |
| Last update date |
Feb 12, 2009 |
| Contact name |
Kathleen M Eyster |
| E-mail(s) |
Kathleen.Eyster@usd.edu
|
| Organization name |
University of South Dakota
|
| Department |
Basic Biomedical Sciences
|
| Street address |
414 E. Clark St.
|
| City |
Vermillion |
| State/province |
SD |
| ZIP/Postal code |
57069 |
| Country |
USA |
| |
|
| Platform ID |
GPL2890 |
| Series (1) |
| GSE4782 |
The effects of estrogen and testosterone on gene expression in the rat mesenteric arteries |
|
