|
| Status |
Public on Jan 31, 2013 |
| Title |
lcn2 knockout, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
lcn2 knockout bone marrow neutrophils
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57bl/6
tissue: bone marrow neutrophil
gender: female
age: 10 weeks
|
| Treatment protocol |
Bone marrow from tibia and femur were flushed with cold HBSS buffer without calcium and magnesium. The red blood cells were lysed by ACK lysis buffer at room temperature for 5 minutes. The remaining cells were separated by discontinuous density gradient centrifugation in Percoll. The cells enriched at the interface between 81% and 62% Percoll were recovered for miRNA precipitation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs containing miRNAs were isolated using mirVana PARIS kit (Life Technology)
|
| Label |
biotin
|
| Label protocol |
One microgram of total RNA was labeled using the Flash Tag Biotin HSR kit (Genisphere)
|
| |
|
| Hybridization protocol |
Affymetrix standard procedure
|
| Scan protocol |
Affymetrix standard procedure
|
| Description |
miRNA expression from bone marrow neutrophils of female mice at the age of 10 weeks
|
| Data processing |
RMA-DABG signal intensity calculation expressed in log2 scale. We sorted out Mus musculus transcripts out of the 131 organisms covered by the miRNA 2.0 array.
program-name = Expression Console
program-version = 1.2.0.20
|
| |
|
| Submission date |
Jan 30, 2013 |
| Last update date |
Jan 31, 2013 |
| Contact name |
Zhuoming Liu |
| Organization name |
Case Western Reserve University
|
| Street address |
2103 Cornell Road
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE43890 |
MiRNA expression profiling in bone-marrow-derived neutrophils of lcn2 deficient mouse |
| GSE43891 |
Expression profiling in bone-marrow-derived neutrophils of lcn2 deficient mouse |
|
