|
| Status |
Public on Aug 02, 2013 |
| Title |
peripheral blood T-cells mRNA Patient9 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood T-cell
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: multiple sclerosis
cell type: peripheral blood T-cells
|
| Treatment protocol |
Cells were snap frozen in -80 C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation,15 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
After hybridisation (according to the Minimum Information About a Microarray Experiment guideline, the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
|
| Description |
gene expression data from T-cell
|
| Data processing |
The raw expression signals were preprocessed following the method of Probe Logarithmic Intensity Error (PLIER) based on perfect match only (pm-only) calculation (Affymetrix).The robust quantile method was applied to get normalized expression values using the Affymetrix software expression console.
|
| |
|
| Submission date |
Jan 17, 2013 |
| Last update date |
Aug 02, 2013 |
| Contact name |
Intawat Nookaew |
| E-mail(s) |
inookaew@uams.edu
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Biomedical Informatics
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE43591 |
MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS) mRNA |
| GSE43592 |
MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS) |
|
