|
| Status |
Public on Jan 15, 2013 |
| Title |
A549 cisplatin-sensitive non-small cell lung cancer cell line_microRNA_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
parental A549 lung cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549 (parental)
cell type: lung adenocarcinoma cells
cisplatin sensitivity: sensitive
|
| Growth protocol |
Cells were maintained in RPMI-1640 medium (Life Technologies) supplemented with 10% fetal calf serum (Gibco, Grand Island, NY, USA) in a humid atmosphere containing 5% CO2 at 37°C. The A549/CDDP cell medium additionally contained 2 mg/L cisplatin in order to maintain its drug-resistant phenotype.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
miRNA microarray target labeling was conducted using 1000 ng of total RNA and the FlashTag Biotin HSR kit (Genisphere, Hatfield, PA).
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
microRNA expression data of A549 rep 2.
|
| Data processing |
Background detection was followed by RMA global background correlation, quantile normalization, median estimation and log2-transformation using the miRNA QC software tool (Affymetrix).
|
| |
|
| Submission date |
Jan 02, 2013 |
| Last update date |
Jan 15, 2013 |
| Contact name |
Yong Yang |
| E-mail(s) |
yyxyz19840408737@sina.com
|
| Organization name |
ccmu
|
| Street address |
baijiazhuang street 8
|
| City |
Beijing |
| ZIP/Postal code |
100069 |
| Country |
China |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE43249 |
Noncoding RNA expression profiling of cisplatin-resistant cells derived from the A549 lung cell line (miRNA) |
| GSE43494 |
Noncoding RNA expression profiling of cisplatin-resistant cells derived from the A549 lung cell line |
|
