NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1057948 Query DataSets for GSM1057948
Status Public on Jan 22, 2013
Title peripheral blood T-cells Control5 [mRNA]
Sample type RNA
 
Source name peripheral blood T-cell
Organism Homo sapiens
Characteristics group: Control
Treatment protocol Cells were snap frozen in -80 C
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation,15 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol After hybridisation (according to the Minimum Information About a Microarray Experiment guideline, the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
Description gene expression data from T-cell
Data processing The raw expression signals were preprocessed following the method of Probe Logarithmic Intensity Error (PLIER) based on perfect match only (pm-only) calculation (Affymetrix).The robust quantile method was applied to get normalized expression values.
 
Submission date Dec 28, 2012
Last update date Sep 01, 2016
Contact name Intawat Nookaew
E-mail(s) inookaew@uams.edu
Organization name University of Arkansas for Medical Sciences
Department Department of Biomedical Informatics
Street address 4301 W Markham St
City Little Rock
ZIP/Postal code 72205
Country USA
 
Platform ID GPL570
Series (2)
GSE43177 MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP) [mRNA]
GSE43179 MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP)
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE normalized expression values

Data table
ID_REF VALUE
1007_s_at 5.923796819
1053_at 6.353281212
117_at 5.254968018
121_at 5.557892249
1255_g_at 4.88362625
1294_at 8.465402222
1316_at 6.793812048
1320_at 4.95058501
1405_i_at 9.768969684
1431_at 4.986758826
1438_at 5.154770627
1487_at 6.671985921
1494_f_at 4.904927003
1552256_a_at 5.154248667
1552257_a_at 8.144860333
1552258_at 5.08154603
1552261_at 5.039180418
1552263_at 5.458821176
1552264_a_at 6.143566345
1552266_at 4.80820516

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1057948_C5blood_HG-U133_Plus_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap