|
| Status |
Public on Jun 08, 2013 |
| Title |
parental2 [expression] |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
genotype/variation: parental
|
| Growth protocol |
Cells were cultured in McCoy's 5A medium supplemented with 10% FBS and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNeasy kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
WT Target Labeling Assay was performed using 100ng of total RNA accorind to Affymetrix's 100 ng Total RNA Labeling Protocol.
|
| |
|
| Hybridization protocol |
The chips were hybridized by Affymetrix Hybridization Oven 640 and washed by Affymetrix Fluidics Station 450 according to manufacturer's protocols.
|
| Scan protocol |
The chips were scanned by Affymetrix scanner 3000 7G using manufacturer's default settings.
|
| Description |
SAMPLE 2
|
| Data processing |
Raw Affymetrix intensity measurements of all probe sets were background-corrected, normalized, and summarized into gene level expression measurements using RMA method implemented in Partek Genomic Suite V6.0.
|
| |
|
| Submission date |
Nov 15, 2012 |
| Last update date |
Oct 16, 2018 |
| Contact name |
Charles David Warden |
| Organization name |
City of Hope
|
| Department |
Department of Molecular and Cellular Biology
|
| Street address |
1500 East Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE42307 |
COHCAP HCT116 Mutant Comparison [expression] |
| GSE42310 |
COHCAP: City of Hope CpG Island Analysis Pipeline |
|
