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| Status |
Public on Jun 10, 2017 |
| Title |
Gene expression data from primary human hepatocytes treated with tolvaptan for 4, 24, or 72 h |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
A micropatterned coculture model (HepatoPac®) was used to explore the possibility that changes in exosome content precede overt necrosis in response to tolvaptan, a drug known to cause idiosyncratic drug-induced liver injury. Hepatocytes from 3 human donors were exposed to a range of tolvaptan concentrations bracketing plasma Cmax (0.01 - 50 µM) or DMSO control continuously for 4, 24, or 72 h.
While ALT release was not significantly affected, tolvaptan exposure resulted in increased release of exosomal microRNA-122 (miR-122) into the medium. Microarray analysis was used to idenfity the activation of molecular stress response pathways most associated with increases in exosomal miR-122.
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| Overall design |
54 samples were analyzed representing N=1 per donor, time point, treatment, and concentration
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| Contributor(s) |
Mosedale M |
| Citation(s) |
29029277 |
| Submission date |
Jun 09, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Merrie Mosedale |
| Organization name |
UNC Eshelman School of Pharmacy
|
| Department |
Division of Pharmacotherapy and Experimental Therapeutics
|
| Lab |
Institute for Drug Safety Sciences
|
| Street address |
301 Pharmacy Lane, Kerr Hall CB#7569
|
| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA389817 |
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