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| Status |
Public on May 19, 2017 |
| Title |
Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To elucidate the molecular bases for the infertile phenotype of Zmym3 KO mice at the mRNA expression level, we carried out RNA sequencing (RNA-seq) analysis on cultured spermatogonial stem cells (SSCs), c-KIT+ pre-meiotic cells induced from SSCs and spermatocytes directly isolated from adult mouse testes.
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| |
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| Overall design |
The cells used for RNA-Seq analysis including SSCs from Zmym3 WT and KO mice (SSC:WT and SSC:KO), c-KIT+ pre-meiotic cells induced from SSCs (c-KIT+ WT and c-KIT+:KO) and spermatocytes from 5- and 7-month mice (5m-SC:WT, 5m-SC:KO, 7m-SC:WT and 7m-SC:KO).
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| |
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| Contributor(s) |
Hu X, Lin X, Han C |
| Citation(s) |
28661483 |
| Submission date |
May 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Chunsheng Han |
| E-mail(s) |
hancs@ioz.ac.cn
|
| Phone |
86-10-64807105
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Zoology
|
| Lab |
State Key Laboratory of Reproductive Biology
|
| Street address |
1-5 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
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| Platforms (2) |
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| Samples (12)
|
| GSM2631949 |
Zmym3 KO SSC replicate 2 |
| GSM2631950 |
Zmym3 WT c-KIT+ pre-meiotic cells replicate 1 |
| GSM2631951 |
Zmym3 WT c-KIT+ pre-meiotic cells replicate 2 |
| GSM2631952 |
Zmym3 KO c-KIT+ pre-meiotic cellsreplicate 1 |
| GSM2631953 |
Zmym3 KO c-KIT+ pre-meiotic cellsreplicate 2 |
| GSM2631954 |
Spermatocytes from 5-month Zmym3 WT mice |
| GSM2631955 |
Spermatocytes from 5-month Zmym3 KO mice |
| GSM2631956 |
Spermatocytes from 7-month Zmym3 WT mice |
| GSM2631957 |
Spermatocytes from 7-month Zmym3 KO mice |
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| Relations |
| BioProject |
PRJNA387186 |
| SRA |
SRP107342 |
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