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| Status |
Public on Oct 31, 2018 |
| Title |
The effect of TAZ-K in neonatal rat cardiomyocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Accumulating evidence suggests that new cardiomyocytes are generated from existing cardiomyocytes at a low rate throughout life. This limited regenerative capacity is unable to restore cardiac function after substantial cardiac damage; thus, small molecules have been examined for their ability to enhance the replication of existing cardiomyocytes. A novel compound TAZ-K promoted cardiomyocyte proliferation, therefore, we analyzed the effect of TAZ-K on gene expression in neonatal rat cardiomyocytes. Gene expression involved in cell cycle, anti-oxidant and anti-apoptosis was up-regulated. Thus, TAZ-K may have multifaceted effects and provide a treatment strategy that complements current therapies for patients with myocardial infarction.
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| Overall design |
Primary neonatal rat cardiomyocytes were cultured in DMEM media supplemented with 10% FCS. After 24 hours, cardiomyocytes were starved (0.1% FCS) for 6 hours, subsequently treated with vehicle (3 samples) or TAZ-K (10 µM; 3 samples) for 24 hours. Cardiomyocytes were harvested, and total RNA was isolated and utilized for gene expression analysis.
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| Contributor(s) |
Hara H, Takeda N, Nomura S, Aburatani H, Komuro I |
| Citation |
Hironori Hara, Norifumi Takeda, Masaki Kondo, Mio Kubota, Tatsuo Saito, Junichi Maruyama, Takayuki Fujiwara, Sonoko Maemura, Masamichi Ito, Atsuhiko T. Naito, Mutsuo Harada, Haruhiro Toko, Seitaro Nomura, Hidetoshi Kumagai, Yuichi Ikeda, Hiroo Ueno, Eiki Takimoto, Hiroshi Akazawa, Hiroyuki Morita, Hiroyuki Aburatani, Yutaka Hata, Masanobu Uchiyama, and Issei Komuro. Discovery of a Small Molecule to Increase Cardiomyocytes and Protect the Heart After Ischemic Injury. JACC: Basic to Translational Science 2018;3(5):639-653. DOI:10.1016/j.jacbts.2018.07.005
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| Submission date |
Feb 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Seitaro Nomura |
| E-mail(s) |
senomura-cib@umin.ac.jp
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| Phone |
81338155411
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| Organization name |
The University of Tokyo
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| Department |
Department of Cardiovascular Medicine
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| Street address |
7-3-1, Hongo, Bunkyo-ku
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| City |
Tokyo |
| State/province |
Select a State or Province |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA376527 |
| SRA |
SRP100614 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE95274_processed_data.txt.gz |
340.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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