|
| Status |
Public on Sep 05, 2017 |
| Title |
Transcriptomics analysis of gene expressions and DNA 5mC enrichment levels in control and dnmt1 morphants zebrafish embryos |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
RNA and whole-genome DNA were isolated from control and dnmt1 morphants zebrafish cells using the TRIzol (Tiangen) reagent and phenol-chloroform extraction by following the manual, respectively. The cDNA library for RNA-seq and DNA library for MeDIP-seq were sequenced using HiSeq2000 (Illumina) in single-end read and paired-read mode, reapectively.
|
| |
|
| Overall design |
Examination of gene expressive levels and 5mC enrichment levels in normal and dnmt1 morphants zebrafish cells
|
| |
|
| Contributor(s) |
Wang L, Liu F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Lu WANG |
| E-mail(s) |
wanglu@ioz.ac.cn
|
| Organization name |
INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
|
| Street address |
1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (6)
|
| GSM2465057 |
control zebrafish embryos MeDIP rep1 |
| GSM2465058 |
control zebrafish embryos MeDIP rep2 |
| GSM2465059 |
dnmt1 morphants zebrafish embryos MeDIP rep1 |
| GSM2465060 |
dnmt1 morphants zebrafish embryos MeDIP rep2 |
| GSM2465061 |
control zebrafish embryos transcriptome |
| GSM2465062 |
dnmt1 morphants zebrafish embryos transcriptome |
|
| Relations |
| BioProject |
PRJNA362849 |
| SRA |
SRP097608 |
Less...
More...