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| Status |
Public on Nov 17, 2016 |
| Title |
Whole Genome Bisulfite sequencing for liver DNA methylation analysis of rat exposed to 0.05% Pb |
| Organism |
Rattus norvegicus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We generated 4 Gb of high-quality sequencing data (~1 Gb per sample). The NCD+0.05%Pb group had hypermethylation at the whole genome level, compared to NCD. Difference analysis in gene methylation aimed at TSS region indicated that a total of 2733 genes experienced DNA methylation changes, with 2320 hypermethylated genes and 413 hypomethylated genes of the NCD+0.05%Pb group compared with the NCD group.
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| Overall design |
8-week-old Wistar rats were divide into NCD and NCD+0.05%Pb groups. Rats of the NCD group were treated normal dringking water,rats of NCD+0.05%Pb group were exposed to 0.05% dosages of lead acetate through drinking water.two liver samples were taken out from each group for WGBS,and the DNA methylation level of livers were analyzed and compared between the two groups.
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| Contributor(s) |
Sun H, Lu Y |
| Citation(s) |
28107465 |
| Submission date |
Nov 16, 2016 |
| Last update date |
Feb 15, 2020 |
| Contact name |
Sun hong ling |
| E-mail(s) |
694115677@qq.com
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| Phone |
15000027837
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| Organization name |
Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine
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| Department |
Institute and Department of Endocrinology and Metabolism
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| Lab |
he lab of Endocrinology and Metabolism
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| Street address |
No.639 Zhizaoju Raod , Shanghai
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| City |
Shanghai |
| ZIP/Postal code |
200011 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA354305 |
| SRA |
SRP093620 |
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