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| Status |
Public on Aug 01, 2007 |
| Title |
MDM from COPD patients and healthy subjects after treatment with LPS or fine and ultrafine particles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and healthy subjects was investigated. MDM were treated with LPS, a combination of fine TiO2 and ultrafine Printex90 particles, or remained untreated.
Keywords: disease state analysis
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| Overall design |
MDM of 13 COPD patients and 13 healthy subjects were incubated for four hours with LPS (10ng/ml), a combination of fine TiO2 and ultrafine Printex90 (32ug/ml each) or remained untreated. Cells were harvested, counted, and total RNA was isolated (phenol-chloroform extraction) for each subject individually. Total RNA of the 13 individuals from each group (COPD, healthy) and incubation (untreated, LPS, particles) were pooled and hybridized on a seperate array.
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| Contributor(s) |
Hofer TP, Frankenberger M, Mages J, Lang R, Meyer P, Hoffmann R, Ziegler-Heitbrock L |
| Citation(s) |
18084737 |
| Submission date |
Jul 27, 2007 |
| Last update date |
Jul 15, 2019 |
| Contact name |
Thomas Hofer |
| E-mail(s) |
hofer@helmholtz-muenchen.de
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| Organization name |
Helmholtz Zentrum Munich
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| Department |
Comprehensive Pneumology Center
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| Street address |
Max-Lebsche-Platz 31
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| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA101779 |
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