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| Status |
Public on Jul 21, 2007 |
| Title |
Gene expression profilie during cell cycle in T98G cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The mammalian Retinoblastoma (RB) family including pRB, p107, and p130 represses E2F target genes through mechanisms that are not fully understood. In D. melanogaster, RB-dependent repression is mediated in part by the multisubunit protein complex Drosophila RBF, E2F, and Myb (dREAM) that contains homologs of the C. elegans synthetic multivulva class B (synMuvB) gene products. Using an integrated approach combining proteomics, genomics, and bioinformatic analyses, we identified a p130 complex termed DP, RB-like, E2F, and MuvB (DREAM) that contains mammalian homologs of synMuvB proteins LIN-9, LIN-37, LIN-52, LIN-54, and LIN-53/RBBP4. DREAM bound to more than 800 human promoters in G0 and was required for repression of E2F target genes. In S phase, MuvB proteins dissociated from p130 and formed a distinct submodule that bound MYB. This work reveals an evolutionarily conserved multisubunit protein complex that contains p130 and E2F4, but not pRB, and mediates the repression of cell cycle-dependent genes in quiescence.
Keywords: Gene expression analysis during cell cycle
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| Overall design |
Gene expression during the cell cycle in T98G cells. Cells were serum starved for 72 hours to induce G0 and then restimuated to enter cell cycle by serum addition. BrdU and FACS was done simultaneously to confirm their cell cycle phase.
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| Contributor(s) |
Litovchick L, Sadasivam S, Zhu X, Velmurugan S, Liu XS, DeCaprio JA |
| Citation(s) |
17531812 |
| Submission date |
Jul 20, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Subhashini Sadasivam |
| E-mail(s) |
subhashini.sadasivam@gmail.com
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| Organization name |
Dana Farber Cancer Insitute
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| Department |
Medical Oncology
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| Lab |
Laboratory of James DeCaprio, Mayer 444
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| Street address |
450 Brookline Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| GSM211868 |
RNA from actively growing T98G cells |
| GSM211869 |
RNA from G0 arrested T98G cells |
| GSM211870 |
RNA from T98G cells in G1 stage of the cell cycle |
| GSM211871 |
RNA from T98G cells in S phase of the cell cycle |
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| Relations |
| BioProject |
PRJNA101675 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8537_RAW.tar |
20.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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