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| Status |
Public on Apr 30, 2016 |
| Title |
Temporal comparison of transcriptomic alterations in human, mouse and rat primary B lymphocytes exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) |
| Organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq was used to analyze and compare temporal TCDD-induced gene expression changes in primary human, mouse and rat B cells. Most of the differentially expressed genes exibited species-specific expression. Cross-species comparison identified 28 orthologs commonly deregulated in all 3 species. The study provides new insight into mechanisms of TCDD-induced suppression of B cell effector function.
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| Overall design |
Primary human, mouse, and rat mRNA profiles of primary B cells treated with either vehicle (0.02% DMSO) or 30nM TCDD for 4, 8, and 24h were generated by sequencing. Human B cell treatments was performed in triplicate while primary B cells from 20 mice or 6 rats were pooled.
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| Contributor(s) |
Kovalova N, Nault R, Zacharewski TR, Kaminski NE, Crawford R |
| Citation(s) |
27913140 |
| Submission date |
Apr 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Norb Kaminski |
| E-mail(s) |
kamins11@msu.edu
|
| Phone |
5175289884
|
| Organization name |
MSU
|
| Street address |
1129 Farm Ln, rm. 248
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
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| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA320107 |
| SRA |
SRP074202 |
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