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| Status |
Public on Jun 09, 2007 |
| Title |
Expression profiling of pancreas development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Development of the pancreas from the endoderm is initiated at embryonic day 9 of mouse development and over the following days several different cell types develop from pancreas progenitor cells. A distinct phase of pancreas development, known as the secondary transition, is initiated at day 13 of development and one of the key features of this transition is a massive increase in the number of mature endocrine cells. To study gene expression in pancreas during the secondary transition we performed high-density oligonucleotide microarray experiments on dorsal pancreas tissue isolated from NMRI embryos on consecutive days from e12.5 to e16.5.
Keywords: time course
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| Overall design |
Dorsal pancreata were isolated from embryos at embryonic day 12.5, 13.5, 14.5, 15.5, and 16.5 and pooled litter-wise prior to total RNA extraction. From each pool, two independent labelling reactions were made, and each sample was hybridized to the entire Murine Genome U74 version 2 chip set (A, B, and C).
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| Contributor(s) |
Petri A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 08, 2007 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Andreas Petri |
| E-mail(s) |
andp@novonordisk.com
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| Organization name |
Novo Nordisk A/S
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| Department |
Molecualr Genetics
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| Street address |
Novo Nordisk Park D6 2.154
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| City |
Maaloev |
| ZIP/Postal code |
2760 |
| Country |
Denmark |
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| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
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| Samples (30)
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| GSM199446 |
Pancreas at e13.5, Chip A, rep 2 |
| GSM199447 |
Pancreas at e14.5, Chip A, rep 1 |
| GSM199448 |
Pancreas at e14.5, Chip A, rep 2 |
| GSM199449 |
Pancreas at e15.5, Chip A, rep 1 |
| GSM199450 |
Pancreas at e15.5, Chip A, rep 2 |
| GSM199451 |
Pancreas at e16.5, Chip A, rep 1 |
| GSM199452 |
Pancreas at e16.5, Chip A, rep 2 |
| GSM199453 |
Pancreas at e12.5, Chip B, rep 1 |
| GSM199454 |
Pancreas at e12.5, Chip B, rep 2 |
| GSM199455 |
Pancreas at e13.5, Chip B, rep 1 |
| GSM199456 |
Pancreas at e13.5, Chip B, rep 2 |
| GSM199457 |
Pancreas at e14.5, Chip B, rep 1 |
| GSM199458 |
Pancreas at e14.5, Chip B, rep 2 |
| GSM199459 |
Pancreas at e15.5, Chip B, rep 1 |
| GSM199460 |
Pancreas at e15.5, Chip B, rep 2 |
| GSM199461 |
Pancreas at e16.5, Chip B, rep 1 |
| GSM199462 |
Pancreas at e16.5, Chip B, rep 2 |
| GSM199463 |
Pancreas at e12.5, Chip C, rep 1 |
| GSM199464 |
Pancreas at e12.5, Chip C, rep 2 |
| GSM199465 |
Pancreas at e13.5, Chip C, rep 1 |
| GSM199466 |
Pancreas at e13.5, Chip C, rep 2 |
| GSM199467 |
Pancreas at e14.5, Chip C, rep 1 |
| GSM199468 |
Pancreas at e14.5, Chip C, rep 2 |
| GSM199469 |
Pancreas at e15.5, Chip C, rep 1 |
| GSM199470 |
Pancreas at e15.5, Chip C, rep 2 |
| GSM199471 |
Pancreas at e16.5, Chip C, rep 1 |
| GSM199472 |
Pancreas at e16.5, Chip C, rep 2 |
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| Relations |
| BioProject |
PRJNA100907 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8070_RAW.tar |
79.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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