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| Status |
Public on Jun 01, 2016 |
| Title |
TGFβ signaling directs serrated adenomas to the mesenchymal colorectal cancer subtype [organoids] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of this study was to determine the effects of TGFβ at the premalignant stage of CRC development.
Organoid cultures were isolated from normal colon and from tubular adenomas. One normal colon culture was genetically engineered using the CRISPR-Cas9 system to carry the BRAFV600E mutation.
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| Overall design |
Organoid cultures of three different tubular adenomas and two normal colon samples, and one BRAFV600E-mutant organoid culture received TGFβ or control treatment after which gene expression was analyzed by microarray (Affymetrix U133+ PM Genechip Array).
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| Contributor(s) |
Fessler E, Drost J, van Hooff SR, Linnekamp JF, Wang X, Jansen M, De Sousa E Melo F, Prasetyanti PR, Ijspeert JE, Franitza M, Nürnberg P, van Noesel CJ, Dekker E, Vermeulen L, Clevers H, Medema J |
| Citation(s) |
27221051 |
| Submission date |
Mar 21, 2016 |
| Last update date |
Apr 20, 2018 |
| Contact name |
Sander Roeland van Hooff |
| Organization name |
AUMC
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| Department |
Center for Experimental and Molecular Medicine (CEMM),
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| Lab |
Laboratory for Experimental Oncology and Radiobiology (LEXOR)
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| Street address |
Meibergdreef 9
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| City |
Amsterdam |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE79462 |
TGFβ signaling directs serrated adenomas to the mesenchymal colorectal cancer subtype |
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| Relations |
| BioProject |
PRJNA315876 |
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