|
| Status |
Public on Mar 22, 2016 |
| Title |
Genome-wide microRNA expression profiling in tunicamycin treated NIH3T3 cells |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
NIH 3T3 cells were challenged with tunicamycin for 2, 5 or 10h. MICRO-RNAs that are upregulated or down regulated were identified by micro-ARRAY.
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| |
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| Overall design |
NIH 3T3 cell were challenged with 2ug/ml tunicamycin for 2, 5 or 10 hrs. Total RNA was purified using miRNeasy Mini Kit (QIAGEN). Microarray was carried out using GeneChip® miRNA Arrays (Affymetrix, CA) and results were analyzed using GENESPRING software. ANOVA and T-tests were used to calculate fold change and p-values. Spotfire (Somerville, MA) software was used to generate heat maps.
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| Contributor(s) |
Diehl JA, Bu Y, Chitnis N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P01 CA165997 |
Micro-RNA-dependent regulation of the UPR |
UNIVERSITY OF PENNSYLVANIA |
DIEHL |
|
| Submission date |
Mar 21, 2016 |
| Last update date |
May 02, 2017 |
| Contact name |
John Alan Diehl |
| E-mail(s) |
diehl@musc.edu
|
| Organization name |
Medical University of South Carolina
|
| Street address |
86 Jonathan Lucas Street
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (12)
|
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| Relations |
| BioProject |
PRJNA315869 |
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