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| Status |
Public on Jan 01, 2024 |
| Title |
Dynamics of NRSF/REST motif evolution favor the canonical NRSE/RE1 form |
| Organisms |
Canis lupus familiaris; Equus caballus; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The transcription factor NRSF/REST represses many vertebrate neuronal genes in non-neuronal cells by binding to 3 distinct motif classes, which are the canonical 21bp NRSEs, longer non-canonical sites and solo half-sites. We used ChIP-seq in four mammalian species to determine the evolution of the NRSF binding repertoire. We show that while some NRSEs are deeply conserved, genes with several NRSEs show evidence of compensatory site turnover, suggesting that the association of the transcription factor to its target gene is more important than the specific binding site. We also found that many newborn sites in human are associated with primate specific indels and transposable elements. Our analysis of sites with conserved ChIP-binding in all 4 species demonstrates that both the non-canonical and solo half-sites convert preferentially to canonical motifs. These findings support a model of dynamic conversion between different motif types that account for the preferential accumulation of the canonical NRSE during evolution.
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| Overall design |
Performed NRSF/REST ChIP-seq on mouse macrophages, dog myelomonocytic leukemia cells and horse skin fibroblasts. Each group with two IP replicates and one DNA input.
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| Contributor(s) |
Jiang S, El-Ali N, Ramirez R, Mortazavi A |
| Citation missing |
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| Submission date |
Feb 03, 2016 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Shan Jiang |
| E-mail(s) |
jiangs2@uci.edu
|
| Organization name |
UNIVERSITY OF CALIFORNIA, IRVINE
|
| Lab |
Mortazavi Lab
|
| Street address |
2300E Biological Sciences III
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| City |
IRVINE |
| ZIP/Postal code |
92697 |
| Country |
USA |
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| Platforms (3) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21400 |
Illumina NextSeq 500 (Canis lupus familiaris) |
| GPL21401 |
Illumina NextSeq 500 (Equus caballus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA310756 |
| SRA |
SRP069246 |
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