|
| Status |
Public on Jun 01, 2016 |
| Title |
Small RNA and mRNA expression profiling during zebrafish caudal fin regeneration |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Previous studies of zebrafish caudal fin regeneration have shown that multiple genetic programs are moduled through regulatory factors. MicroRNAs are short highly conserved non-coding genes that suppress expression of target genes and thereby control multiple genetic programs. Given their important regulatory roles and evolutionary conservation, we hypothesize that microRNAs define a conserved genetic regulatory circuit important for appendage regeneration. We characterized microRNA expression during zebrafish caudal fin regeneration using small RNA sequencing. The stages of caudal fin regeneration were assayed for mRNA expression using mRNA sequencing.
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| |
|
| Overall design |
Small RNA and mRNA gene expression profiling during 0 and 4 days post amputation.
|
| |
|
| Contributor(s) |
King BL, Yin VP |
| Citation(s) |
27355827 |
| Submission date |
Oct 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Benjamin L King |
| E-mail(s) |
benjamin.l.king@maine.edu
|
| Phone |
207-581-2803
|
| Organization name |
University of Maine
|
| Department |
Molecular and Biomedical Sciences
|
| Street address |
5735 Hitchner Hall
|
| City |
Orono |
| State/province |
ME |
| ZIP/Postal code |
04469 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA300314 |
| SRA |
SRP065355 |
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