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| Status |
Public on Dec 01, 2007 |
| Title |
Whole genome survey of the obesity model lean congenic mice B6.C-D7Mit353 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have analyzed the differential expression between the obesity model lean congenic B6.C-D7Mit353 (1) and the background C57BL/6J strains, in four different tissues of male mice: Brain, GWAT (Gonadal White Adipose Tissue), liver and muscle (gastrocnemius). From the 32,381 mouse genes covered by the Applied Biosystems AB1700 microarray system, we identified 330 genes passing FDR at 5% and 188 genes passing FDR at 1%, in any one of the four tissues examined. None of the genes that passed FDR testing were in the congenic donor region. 65 genes from the 8 megabases congenic region were assayed; only six genes (Thrsp, Kctd14, Myo7a, Capn5, Dgat2 and Mtap6) passed statistical significance filtering but not FDR filtering. (1) Diament & Warden 2004: PMID 14569280.
Keywords: Congenic mice whole genome gene expression survey
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| Overall design |
Brain, GWAT, liver and muscle RNA were extracted from biological triplicates of the congenic B6.C-D7Mit353 (Brain samples: Br353C1b, Br353C4a, Br353C5b; Liver samples: L571, L409, L552; Adipose samples: GWAT407, GWAT409, GWAT571; Muscle samples: Gas409, Gas407, Gas552) and the background C57BL/6J strains (Brain samples: BrB1b, BrB3b, BrB5b; Liver samples: Lb570, Lb572, Lb573, GWAT572; Adipose samples: GWAT615, GWAT411; Muscle samples: Gas408, Gas615, Gas570). Digoxigenin-UTP labeled cRNA was generated and linearly amplified from 1 ug of total RNA. Array hybridization, Chemiluminescence detection, image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer following the manufacturer's protocol.
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| Contributor(s) |
Perroud B, Sun YA, Blake J, Walden CH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 02, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Yongming Sun |
| E-mail(s) |
yongming.sun@appliedbiosystems.com
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| Phone |
650-638-6879
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| Organization name |
Applied Biosystems
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| Street address |
850 Lincoln Center Dr
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| City |
Foster City |
| State/province |
CA |
| ZIP/Postal code |
94404 |
| Country |
USA |
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| Platforms (1) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA97953 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7431_RAW.tar |
6.1 Mb |
(http)(custom) |
TAR (of TXT) |
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