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| Status |
Public on Jul 03, 2016 |
| Title |
Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish |
| Organisms |
Danio rerio; Oreochromis niloticus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional plasticity is a major driver of phenotypic differences between species. The lower temperature limit (LTL), namely the lower end of survival temperature, is an important trait delimiting the geographical distribution of a species, however, the genetic mechanisms are poorly understood. We investigated the inter-species transcriptional diversification in cold responses between zebrafish Danio rerio and tilapia Oreochromis niloticus, which were reared at a common temperature (28°C) but have distinct LTLs. We identified significant expressional divergence between the two species in the orthologous genes from gills when the temperature cooled to the LTL of tilapia (8°C). Five KEGG pathways were found sequentially over-represented in the zebrafish/tilapia divergently expressed genes in the duration (12 hour) of 8°C exposure, forming a signaling cascade from metabolic regulation to apoptosis via FoxO signaling. Consistently, we found differential progression of apoptosis in the gills of the two species in which zebrafish manifested a delayed and milder apoptotic phenotype than tilapia, corresponding with a lower LTL of zebrafish. We identified diverged expression in 25 apoptosis-related transcription factors between the two species which forms an interacting network with diverged factors involving the FoxO signaling and metabolic regulation. We propose a genetic network which regulates LTL in fishes.
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| Overall design |
Examination of gene expressional divergence in gill between zebrafish and tilapia
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| Contributor(s) |
Hu P, Chen L |
| Citation(s) |
27356472 |
| Submission date |
Jun 17, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Peng Hu |
| E-mail(s) |
penghu41@gmail.com
|
| Organization name |
Shanghai Ocean University
|
| Street address |
Huchenghuan Road 999
|
| City |
Shanghai |
| ZIP/Postal code |
201306 |
| Country |
China |
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| Platforms (2) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
| GPL19474 |
Illumina HiSeq 2000 (Oreochromis niloticus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA287283 |
| SRA |
SRP059605 |
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