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| Status |
Public on Dec 15, 2015 |
| Title |
Evaluation of Inter-Batch Differences in Stem-Cell Derived Neurons |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Genome wide DNA methylation profiling of Neurons derived from human induced pluripotent stem cells (iCell Neurons) at different time points were abtained by the Infinium HumanMethylation450 BeadChip (450K array) Kit (WG-314-1002), which interrogates >480,000 CpG sites in the human genome.In total, 36 samples were profiled using the 450K arrays.
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| Overall design |
1 ug purified DNA from each timepoint was submitted to the University of Chicago Genomics Core where it was converted with the EZ-96 DNA Methylation™ Kit (Deep Well Format), as per manufacturer's protocol, followed by array analysis using the Infinium HumanMethylation450 BeadChip (450K array) Kit (WG-314-1002), which interrogates >480,000 CpG sites in the human genome.
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| Contributor(s) |
Zhang W, Dolan ME |
| Citation(s) |
26774046 |
| Submission date |
Feb 26, 2015 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Wei Zhang |
| E-mail(s) |
wei.zhang1@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
680 N. Lake Shore Drive, Suite 1400
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA276556 |
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