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| Status |
Public on Sep 01, 2015 |
| Title |
miR-200 overexpression in colorectal cancer lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Unsupervised classification of gene expression profiles has resulted in the identification of biologically and clinically distinct colon cancer subtypes (CCSs). The subtype that associates with poor clinical outcome displays a mesenchymal gene expression profile. No driver mutation has been identified for this category and patients are heterogeneous with regard to commonly used clinical markers. Here we report a regulatory network consisting of the miR-200 family members that tunes the majority of genes differentially expressed in the poor prognosis CCS, including genes involved in the epithelial-mesenchymal transition (EMT) process. Our data indicate that the epigenetic silencing of the miR-200 family by promoter methylation is identifying the mesenchymal CCS and is predictive of disease-free survival in this malignancy. We demonstrate that the molecular features of poor prognosis colon cancer - expression of EMT-associated genes and miR-200 promoter methylation - can already be installed at the premalignant stage, suggesting a highly malignant potential of specific colon cancer precursor lesions.
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| Overall design |
Three colorectal cancer cell lines that display methylated miR-200 loci have been used to overexpress miR-200 family members from both loci separatedly or simultaneously.
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| Contributor(s) |
Vermeulen L, Fessler E, Medema JP, Wang X |
| Citation missing |
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| Submission date |
Feb 03, 2015 |
| Last update date |
Apr 20, 2018 |
| Contact name |
Louis Vermeulen |
| E-mail(s) |
l.vermeulen@amc.uva.nl
|
| Phone |
+31 (0)20 56 64824
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| Organization name |
Academic Medical Center
|
| Department |
Center for Experimental Molecular Medicine
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| Lab |
Laboratory for Experimental Oncology
|
| Street address |
Meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1105AZ |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA274343 |
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