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| Status |
Public on Nov 04, 2015 |
| Title |
Genome-wide studies identify a novel interplay between AML1 and AML1/ETO in t(8;21) acute myeloid leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The AML1/ETO fusion protein is essential to the development of acute myeloid leukemia (AML), and is well recognized for its dominant-negative effect on the co-existing wild-type protein AML1. However, the involvement of wild-type AML1 in AML1/ETO-driven leukemogenesis remains elusive. Through chromatin immunoprecipitation sequencing, computational analysis plus a series of experimental validations, we report here that AML1 is able to orchestrate the expression of AML1/ETO targets regardless of being activated or repressed, via forming a complex with AML1/ETO and via recruiting the cofactor.
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| Overall design |
4 ChIP-seq assays were used to identify the high confidence binding regions of AML1-ETO and AML1 in t(8;21) AML Kasumi-1 cell lines. The anti-AML1 (N20) antibody targets the N-terminus of AML1 and recognizes both AML1 and AML1/ETO; the anti-AML1 (C19) antibody targets the C-terminus of AML1 and recognizes AML1 but not AML1/ETO; the anti-ETO (C20) antibody targets the C-terminus of ETO and specifically recognizes AML1/ETO. 2 ChIP-seq assays were used to identify the binding regions of AML1 in human macrophage U937 cell lines. And the total input was used as control.
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| Contributor(s) |
Wang K, Li Y, Wang H |
| Citation(s) |
26546158 |
| Submission date |
Jan 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Huanwei Wang |
| E-mail(s) |
sjtu_whw@sjtu.edu.cn
|
| Phone |
+86-18817821254
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| Organization name |
Rui-Jin Hospital affiliated to Shanghai Jiao Tong University School of Medicine (RJH-SJTU-SM)
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| Department |
Shanghai Institute of Hematology
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| Street address |
197 Ruijin Rd II
|
| City |
shanghai |
| State/province |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA273981 |
| SRA |
SRP052956 |
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