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Series GSE65398 Query DataSets for GSE65398
Status Public on Jan 29, 2015
Title Expression data from human carcinoma (MCF7) derived cells that have been exposed to insulin analogues
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Insulin analogues are designed to improve the pharmacokinetic parameters compared to regular human insulin. This provides a sustained control of blood glucose levels in diabetic patients. All novel insulin analogues are tested for their mitogenic side effects, however these assays do not take into account the molecular mode-of-action of different insulin analogues. Insulin analogues can bind the insulin receptor (INSR) and the insulin-like growth factor-1 receptor (IGF1R) with different affinities and consequently will activate different downstream signaling pathways. Here we used a panel of MCF7 human breast cancer cell lines that selectively express either one of the isoforms of the INSR (IRA or IRB) or the IGF1R. We sought to study the role of the different receptors (IRA, IRB and IGF1R) in the mitogenic signaling of insulin-like molecules (including insulin, glargine, X10 (or AspB10) and IGF1).
 
Overall design MCF7 IRA, MCF7 IRB or MCF7 IGF1R cells (as described in Arch Toxicol. 2014 Apr;88(4):953-66. doi: 10.1007/s00204-014-1201-2. Epub 2014 Jan 25.) were cultured in RPMI supplemented with 5% (v/v) CDFBS (Hyclone) and used for experiments. Cells have been exposed for 1 or 6 hours to 10 nM of the indicated insulin-like molecule. As a control sample a vehicle stimulation was performed that contained everything except the active compound.
 
Contributor(s) Ter Braak B, Wink S, Koedoot E, Pont C, Siezen C, van der Laan JW, van de Water B
Citation(s) 26187749
Submission date Jan 28, 2015
Last update date Nov 06, 2019
Contact name Steven Wink
E-mail(s) s.wink.3@lacdr.leidenuniv.nl
Organization name LACDR
Department Division of Toxicology
Street address Einsteinweg 55
City Leiden
State/province Zuid Holland
ZIP/Postal code 2333CC
Country Netherlands
 
Platforms (1)
GPL13158 [HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate
Samples (96)
GSM1595297 MCF7 IRA exposed for 1 h to insulin replicate 1
GSM1595298 MCF7 IRB exposed for 1 h to insulin replicate 1
GSM1595299 MCF7 IGF1R exposed for 1 h to insulin replicate 1
Relations
BioProject PRJNA273881

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Supplementary file Size Download File type/resource
GSE65398_RAW.tar 196.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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