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| Status |
Public on Jul 01, 2007 |
| Title |
Ovalbumin sensitized and challenged induces differential gene expression in mouse lungs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To gain insight into the promoting effect of ultrafine particle inhalation on development and progression of allergic asthma, we selected an experimental approach involving exposure to ultrafine carbon particles (UCP) and gene expression profiling of lungs from mice with experimental, ovalbumin induced allergy. Comparative gene expression analysis was performed by hybridizing pooled cDNA samples from lavaged lungs of different groups.
These results suggest that allergic sensitization may represent a susceptibility factor for effects of UCP on gene expression in the lung. In sensitized individuals UCP exposure, such as found in polluted air, thus may contribute to the development and /or aggravation of allergic asthma.
Keywords: Particle Inhalation, lung, ovalbumin sensitzed and challanged, experssion profiling
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| Overall design |
Lungs of groups of six non-sensitized, ovalbumin sensitized, or sensitized and ovalbumin challenged BALB/cJ mice, either subjected to particle-free or UCP containing air; two replicates including one dye swap experiment have been performed for lungs: a) non-sensitized particle free air versus sensitized and ovalbumin challenged sensitized particle free air; b) non-sensitized UCP containing air versus sensitized and ovalbumin challenged sensitized UCP containing air
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| Contributor(s) |
Beckers J, Rieger S, Schulz H, Stoeger T |
| Citation(s) |
18300050 |
| Submission date |
Dec 11, 2006 |
| Last update date |
Sep 21, 2012 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
|
| Department |
Institute of Experimental Genetics
|
| Lab |
Gene Regulation & Epigenetics
|
| Street address |
Ingolstaedter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL4636 |
GSF/IEG mouse 20K array version2 |
|
| Samples (4)
|
| GSM149401 |
OVA sensitized and challange_praticle free air_rep1 |
| GSM149402 |
OVA sensitized, OVA challenged_rep2 |
| GSM149403 |
OVA sensitized, OVA challenged, UCP exposure_rep1 |
| GSM149404 |
OVA sensitized, OVA challenged, UCP exposure_rep2 |
|
| Relations |
| BioProject |
PRJNA98667 |
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