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| Status |
Public on Oct 19, 2016 |
| Title |
DNA methylation profile for male SD Rats upon fructose and DHA treatment by RRBS |
| Organism |
Rattus norvegicus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
To investigate the beneficial effects of DHA (omega-3) on affecting fructose induced brain dysfunction and metabolic disorders by sequencing the methylome (RRBS) in the hypothalamus and hippocampus of male SD rats.
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| Overall design |
Male Sprague–Dawley (SD) rats of 2 months old were randomly assigned to an omega-3 fatty acid diet (n-3 diet) with or without fructose added in the drinking water (15%) for six weeks. The rats were sacrificed by decapitation. Hypothalamus and hippocampus were dissected out, flash frozen, and stored at -70°C for later transcriptome and DNA methylome sequencing experiments. All experiments were performed in accordance with the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the University of California at Los Angeles Chancellor’s Animal Research Committee.
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| Contributor(s) |
Meng Q, Yang X, Gomez-Pinilla F |
| Citation(s) |
27322469 |
| Submission date |
Jan 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xia Yang |
| E-mail(s) |
xyang123@ucla.edu
|
| Organization name |
UCLA
|
| Department |
IBP
|
| Street address |
2000 Terasaki Life Sciences Bldg, 610 Charles E. Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE64817 |
Gene expression and methylation profile for male SD Rats upon fructose and DHA treatment |
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| Relations |
| BioProject |
PRJNA271943 |
| SRA |
SRP051852 |
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