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| Status |
Public on Feb 02, 2015 |
| Title |
RNA sequencing of allelic series of miR-17~92 mutant embryos. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the generation of an allelic series of miR-17~92 mutant mice, each carrying the targeted deletion of specific miRNA families encoded by the cluster. We integrate the phenotypic characterization of these strains with RNA-seq analysis showing the consequences of selective miRNA inactivation on gene expression in the developing embryo.
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| Overall design |
We generated and analyzed RNA-seq data from 8 different miR-17~92 alleles, in three different microdissected tissues collected from E9.5 mouse embryos. Datasets contain biological triplicates (except for duplicates in one case, Delta_17_18_92) for a total of 71 samples.
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| Contributor(s) |
Betel D, Han Y, Vidigal JA, Singh I, Gonzalez A, Leslie C, Ventura A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 03, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrea Ventura |
| E-mail(s) |
venturaa@mskcc.org
|
| Phone |
6468883068
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Cancer Biology & Genetics Program
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| Lab |
Ventura
|
| Street address |
408 East 69th, ZRC-1201
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (71)
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| Relations |
| BioProject |
PRJNA269137 |
| SRA |
SRP050491 |
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