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| Status |
Public on Sep 27, 2014 |
| Title |
Identification of genes that are differentially expressed in alt1 and WT |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
we characterized the rice alkaline tolerant mutant, alt1. Map-based cloning revealed that alt1 harbors a mutation in a putative chromatin remodeling ATPase gene. ALT1-RNAi transgenic plants mimicked the alt1 phenotype, exhibiting tolerance to alkali stress in a transcript dosage-dependent manner. We found that the predicted ALT1 protein belonged to the Ris1 subgroup of the Snf2 family and was localized in the nucleus. qRT-PCR analysis showed that ALT1 was predominantly expressed in leaf blades and sheaths, and that ALT1 transcription was rapidly suppressed after alkaline treatment. These results support the notion that ALT1 is a negative regulator of alkaline tolerance. Roots of two-leaf stage alt1 and WT seedlings grown under normal conditions were sampled for microarray analysis. The transcriptomic profiles were investigated using an Agilent-015241 Rice Gene Expression 4×44 K Microarray (Agilent Technology) containing 32,325 probes corresponding to cDNA, 6,934 probes corresponding to expressed sequence tags (ESTs), and 2,612 probes corresponding to gene predicted loci, respectively, with three independent biological replicates.
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| Overall design |
Roots of two-leaf stage alt1 and WT seedlings grown under normal conditions were sampled for microarray analysis
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| Contributor(s) |
Guo M, Yao S |
| Citation(s) |
25473841 |
| Submission date |
Sep 26, 2014 |
| Last update date |
May 01, 2015 |
| Contact name |
Xiaoqiang Liu |
| E-mail(s) |
xqliu@genetics.ac.cn
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| Organization name |
the institute of genetics and developmental biology
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| Department |
Chinese academy of sciences
|
| Street address |
No.1 West Beichen Road,Chaoyang District
|
| City |
Beijing, |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL8852 |
Agilent-015241 Rice Gene Expression 4x44K Microarray (Probe name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA262135 |
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