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| Status |
Public on Aug 09, 2014 |
| Title |
The Nuclear Exosome is Active and Important during Budding Yeast Meiosis |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have analysed the activity of the nuclear exosome during meiosis by deletion of TRF4, which encodes a key component of the exosome targeting complex TRAMP. We find that TRAMP mutants produce high levels of CUTs during meiosis that are undetectable in wild-type cells, showing that the nuclear exosome remains functional for CUT degradation. Lack of TRAMP activity stabilises ~1600 CUTs in meiotic cells, which occupy 40% of the binding capacity of the nuclear cap binding complex (CBC).
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| Overall design |
One sample each of Cbc2-associated RNA from wild-type and trf4-deleted cells at 6 hours of meiosis
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| Contributor(s) |
Frenk S, Oxley D, Houseley J |
| Citation(s) |
25210768 |
| Submission date |
Aug 08, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jonathan Houseley |
| E-mail(s) |
jon.houseley@babraham.ac.uk
|
| Organization name |
Babraham Institute
|
| Department |
Epigenetics Programme
|
| Street address |
Babraham Institute
|
| City |
Babraham |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB22 3AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13272 |
Illumina Genome Analyzer IIx (Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA257783 |
| SRA |
SRP045356 |
