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| Status |
Public on Oct 01, 2015 |
| Title |
Genome-wide analysis of H3K27me3 in Ezh2-conditional iMEFs |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We analyzed the genomic distribution of H3K27me3 in a clone of c-Myc iMEFs (clone C2) either i) wild-type for Ezh2, ii) in the presence of overexpressed exogenous Ezh2, iii) Ezh2-mutant, and iv) Ezh1/Ezh2 pre-deletion (Ezh1/Ezh2 introduced before deletion of endogenous Ezh2) and Ezh2 post-deletion rescue (Ezh2 re-introduced in Ezh2-mutant cells).
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| Overall design |
Experiment 1: H3K27me3 ChIP-seq in C2 iMEFs either Ezh2 WT, Ezh2-mutant or Ezh2 post-rescue condition. Experiment 2: H3K27me3 ChIP-seq in C2 iMEFs either Ezh2 WT, Ezh2-mutant, in the presence of overexpressed Ezh2, in Ezh1 pre-rescue condition and in Ezh2 pre-rescue condition.
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| Contributor(s) |
Teissandier A, Margueron R, Wassef M |
| Citation(s) |
26637281 |
| Submission date |
Jul 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Servant |
| E-mail(s) |
Nicolas.Servant@curie.fr
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| Organization name |
Institut Curie
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| Street address |
26 rue d'ulm
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| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
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| Platforms (2) |
| GPL16790 |
AB 5500 Genetic Analyzer (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE59427 |
Impaired PRC2 activity promotes transcriptional instability and favors breast tumorigenesis |
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| Relations |
| BioProject |
PRJNA255242 |
| SRA |
SRP044309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59293_RAW.tar |
1.6 Gb |
(http)(custom) |
TAR (of BED, BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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