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| Status |
Public on Sep 01, 2014 |
| Title |
Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birth, induces metabolic alterations and affects ileal expression of genes involved in immunity. LDP that is limited to early life transiently perturbs the microbiota, which is sufficient to induce sustained effects on body composition, indicating that microbiota interactions in infancy may be critical determinants of long-term host metabolic effects. In addition, LDP enhances the effect of high-fat diet induced obesity. The growth promotion phenotype is transferrable to germ-free hosts by LDP-selected microbiota, showing that the altered microbiota, not antibiotics per se, play a causal role. These studies characterize important variables in early-life microbe-host metabolic interaction and identify several taxa consistently linked with metabolic alterations.
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| Overall design |
Male and female mice were exposed to low-dose penicillin from birth. In a second experiment, microbiota from female control and LDP mice was transferred to 3-week old female germ-free mice. Livers were collected at 8 weeks of age, RNA was extracted, and transcriptional differences were measured by RNAseq.
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| Contributor(s) |
Cox LM, Yamanishi S, Sohn J, Alekseyenko AV, Leung JM, Cho I, Kim SG, Li H, Gao Z, Mahana D, Zarate Rodriguez JG, Rogers AB, Robine N, Loke P, Blaser MJ |
| Citation(s) |
25126780 |
| Submission date |
May 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiho Sohn |
| E-mail(s) |
jihosohn@buffalo.edu
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| Organization name |
SUNY Buffalo
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| Department |
Oral Biology
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| Lab |
Keith Kirkwood
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| Street address |
3435 Main Street, 644 Biomedical Research Building
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| City |
Buffalo |
| State/province |
New York |
| ZIP/Postal code |
14214 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE58087 |
Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences |
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| Relations |
| BioProject |
PRJNA248883 |
| SRA |
SRP042368 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58089_CoxBlaser_NYGC_femaleSamples_count_matrix.txt.gz |
380.0 Kb |
(ftp)(http) |
TXT |
| GSE58089_CoxBlaser_NYGC_maleSamples_count_matrix.txt.gz |
564.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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