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| Status |
Public on May 29, 2014 |
| Title |
Defining Differentiated Methylation Regions Especial for Pluripotency Acquisition and Maintenance in Human Stem Cell |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by genome tiling array
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| Summary |
Here, we determined the DNA methylation profiles of 12 human cell lines, including 2 ESC lines, 2 pESC lines,4 virally-delivered iPSC lines, 2 episomal-delivered iPSC lines, and 2 parent cell lines that iPSCs derived from using Illumina’s Infinium HumanMethylation450. The iPSCs exhibited a hypermethylation status similarly to ESCs but distinct differences from the parent cells. Genes of common methylation pattern between iPSCs and ESCs were regarded as critical factors for stemness, while differences existing between iPSCs and ESCs implied that iPSCs partly retained the parental characteristics and gained de novo methylation aberrances during cell reprogramming. No significant variant was identified between virally- and episomal- systems. De novo differentiated methylation regions as the signature of particular stem cell lines were figured out in detail in this study.
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| Overall design |
2 ESC lines, 2 pESC lines,4 virally-delivered iPSC lines, 2 episomal-delivered iPSC lines, and 2 parent cell lines that iPSCs derived from
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| Contributor(s) |
He W, Fan Y, Sun X |
| Citation(s) |
25250679 |
| Submission date |
May 27, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Wenyin HE |
| E-mail(s) |
31848346@qq.com
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| Phone |
8620-81297081
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| Organization name |
The third affiliated hospital of Guangzhou medical university
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| Street address |
No.63, Duobao road
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510150 |
| Country |
China |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA248741 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57992_RAW.tar |
280.7 Mb |
(http)(custom) |
TAR (of IDAT) |
| GSE57992_raw.signal.txt.gz |
46.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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