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| Status |
Public on Dec 31, 2018 |
| Title |
Expression profiling of MAPK inhibition in WM266-4 BRAF V600D mutant melanoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Extracellular signal regulated kinases, ERK1 and ERK2 are often considered as redundant due to their high homology, large number of overlapping substrates, and ability to substitute for each other in genetically engineered mouse models. We have investigated the individual contribution of ERK1 and ERK2 to the survival of human melanoma cell lines driven by oncogenic BRAF. ERK2, but not ERK1 activity, was crucial to drive survival and maintain transcriptional output of the MAPK pathway. Furthermore, we found that ERK2 DEF-domain interactions were crucial for transcriptional regulation and survival in some cells, but not in others, whereas ERK2-substrate interactions at the D-docking motif were largely dispensable.
We used microarrays to examine the global impact of gene expression by imhibiting different nodes of MAPK pathway.
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| Overall design |
WM266-4 BRAF V600D mutant melanoma cell line was treated with inhibitors to BRAF (LGX818), MEK1/2 (trametinib, MEK162), ERK1/2-inhibitor, or dox-inducible shRNAs targeting ERK1 or ERK2 at 2 different time points.
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| Contributor(s) |
Yu GK, Faure M, Zavorotinskaya T, Stuart D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 16, 2014 |
| Last update date |
Jan 01, 2019 |
| Contact name |
Guoying Karen Yu |
| E-mail(s) |
yu.karen@gmail.com
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| Organization name |
Novartis - NIBR
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| Department |
bioinformatics
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| Street address |
4560 Horton St, MS 4.1
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| City |
Emeryville |
| State/province |
CA |
| ZIP/Postal code |
94608 |
| Country |
USA |
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| Platforms (1) |
| GPL17810 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Brainarray HGU133Plus2_Hs_ENTREZG_v16] |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA247936 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57721_RAW.tar |
277.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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