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| Status |
Public on May 08, 2014 |
| Title |
Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays (GPL14921) |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
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| Summary |
We investigated whether mouse serum autoantibody binding patterns on random-sequence peptide microarrays (immunosignaturing) can be used for diagnosing and predicting the onset of lupus and its central nervous system (CNS) manifestations.
Submitter states "We have no processed data to submit. We have no gpr files to submit."
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| Overall design |
To identify possible predictive and diagnostic peptides of lupus and CNS-lupus, we carried out two studies and selected peptides in common across both studies. In the first study we tested 3-6 MRL/lpr, MRL/mp and C3H/HeJ mice at four months of age. For study two we tested 9-10 MRL/lpr and MRL/mp at 1.5 and 4 months of age. In both studies the mice sera were diluted 1/500 and analyzed using microarray peptides from platform GPL14921. We ran each sample in triplicate. The MRL/lpr and MRL/mp are the autoimmune strains and the C3H/HeJ is the control strain.
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| Contributor(s) |
Williams SM, Stafford P, Hoffman SA |
| Citation(s) |
24908187 |
| Submission date |
May 07, 2014 |
| Last update date |
Aug 07, 2014 |
| Contact name |
Phillip Stafford |
| E-mail(s) |
phillip.stafford@asu.edu
|
| Organization name |
Biodesign Institute
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| Department |
Center for Innovations in Medicine
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| Lab |
Biostatistics
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| Street address |
1001 S. McAllister
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| City |
Phoenix |
| State/province |
AZ |
| ZIP/Postal code |
85287 |
| Country |
USA |
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| Platforms (1) |
| GPL14921 |
ASU_random-sequence peptide microarray |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA246380 |
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