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| Status |
Public on Mar 24, 2015 |
| Title |
Immortalization of T-cells is accompanied by gradual changes in CpG methylation resulting in a profile resembling a subset of T-cell leukemias [methylation] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
We have previously described gene expression changes during spontaneous immortalization of T cells, thereby identifying cellular processes important for cell growth crisis escape and long term proliferation. Here we analyze the same model to investigate the role of genome-wide methylation in the immortalization process at different time points pre- and post- crisis using high-resolution arrays. We show that over time in culture there is an overall accumulation of methylation alterations, with preferential increased methylation close to transcription start sites, island and shore regions. Interestingly, the pattern of CpG site methylation observed in post-crisis T-cell cultures was similar to clinical T-cell acute lymphoblastic leukemia (T-ALL) samples classified as CpG island methylator phenotype positive (CIMP+). These sites were highly overrepresented by polycomb target genes and involved in developmental, cell adhesion and cell signaling processes. The presence of nonrandom methylation events in in vitro immortalized T-cell cultures and diagnostic T-ALL samples indicates altered methylation of CpG sites with a possible role in malignant hematopoiesis.
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| Overall design |
Bisulphite converted DNA from the Cell culture and leukemia samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip
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| Contributor(s) |
Degerman S, Landfors M, Siwicki JK, Revie J, Borssén M, Evelönn E, Forestier E, Chrzanowska KH, Rydén P, Keith WN, Roos G |
| Citation(s) |
25065939 |
| Submission date |
Mar 20, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Sofie Degerman |
| E-mail(s) |
sofie.degerman@umu.se
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| Organization name |
Umeå University
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| Department |
Medical Biosciensces
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| Lab |
Pathology
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| Street address |
NUS, Blg 6M, 2nd floor
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| City |
Umeå |
| State/province |
Sweden |
| ZIP/Postal code |
90185 |
| Country |
Sweden |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE56070 |
Immortalization of T-cells is accompanied by gradual changes in CpG methylation resulting in a profile resembling a subset of T-cell leukemias |
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| Relations |
| BioProject |
PRJNA242337 |
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