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Series GSE5574 Query DataSets for GSE5574
Status Public on Aug 23, 2006
Title Gene expression in PBMC from Multiple Sclerosis patients undergoing interferon beta therapy.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We studied the gene expression patterns in PBMC from relapsing remitting MS patients undergoing weekly IFN-ß-1a therapy. On the basis of two fold changes in expression levels and statistical analyses we selected a diagnostic set of 137 genes that were differentially expressed between pretreatment and IFN-ß-1a-treated MS patients. Some these 137 genes may provide the basis for lack of IFN-ß-1a response.
Keywords: Drug response to Interferon beta therapy in Multiple Sclerosis patients.
 
Overall design We studied five RRMS patients with ages ranging from 32 to 46 years naïve to IFN-ß-1a therapy at the outset of the trial. The pretreatment and acute peripheral blood samples respectively were drawn the day before and the day after the start of the therapy. The chronic samples were drawn one day before the weekly treatment regimen approximately six months following the initiation of therapy. Mononuclear cells were purified from the fresh peripheral blood specimens using Ficoll-Paque density gradient separation. RNA was isolated from the PBMC using the Qiagen column purification procedure (Qiagen, Valencia, CA). We utilized the CodeLink Expression Bioarrays for the analysis of gene expression in PBMC.
 
Contributor(s) Singh MK
Citation(s) 17467740
Submission date Aug 22, 2006
Last update date Mar 16, 2012
Contact name Mandal. K. Singh
E-mail(s) mandal.singh@chp.edu
Phone 412-692-8402
Fax 412-692-6918
Organization name Childrens Hospital of Pittsburgh
Department Transplant
Street address 3460 Fifth Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15206
Country USA
 
Platforms (1)
GPL4191 CodeLink UniSet Human I Bioarray
Samples (36)
GSM129422 PatientDD Pretreatment RNA_rep1 (DD109Pre)
GSM129423 PatientDD Pretreatment RNA_rep2 (DD111Pre)
GSM129424 PatientDD Acute RNA_rep1 (DD112Acute)
Relations
BioProject PRJNA95431

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