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| Status |
Public on Jun 20, 2014 |
| Title |
Effect of lymphotoxin beta receptor blockade on microRNA expression in the spleen [AffyMircoRNA] |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Follicular dendritic cells (FDC) are important stromal cells within the B cell follicles and germinal centres (GC) of secondary lymphoid tissues. FDC trap and retain native antigens on their surfaces in the form of immune complexes which they display to B cells, in order to select those cells with the highest antigen affinity. MicroRNAs are short, non-coding RNAs of approximately 18-25 nucleotides in length that regulate gene expression at the post-transcriptional level by repressing the translation of target genes. In the current study in vivo and in vitro systems were used to identify microRNAs that are differentially expressed as a result of FDC depletion. Constitutive lymphotoxin-β receptor (LTβR) stimulation is required to maintain FDC in their differentiated state. We show that the rapid de-differentiation of spleen FDC that followed LTβR-blockade, coincided with a significant decrease in the expression of mmu-miR-100-5p, mmu-miR-138-5p and mmu-miR-2137. These microRNAs were shown to be expressed in the FDC-like cell line, FL-YB, and specific inhibition of mmu-miR-100-5p significantly enhanced expression of Il6, Ptgs1/2 and Tlr4 in this cell line. The expression of each of these genes by FDC plays an important role in regulating GC size and promoting high-affinity antibody responses, suggesting that mmu-miR-100-5p may help regulate their expression during GC reactions.
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| Overall design |
C57BL/6 mice were given a single intravenous injection of 100 µg of LTβR to temporarily deplete their FDC. At intervals after treatment 4 spleens from each group were harvested and RNA prepared. For each group samples were pooled into 2 groups of 2 and microRNA expression levels compared. Spleens from LTb-/- mice were also analysed.
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| Contributor(s) |
Aungier SR, Ohmori H, Clinton M, Mabbott NA |
| Citation(s) |
24944008 |
| Submission date |
Mar 10, 2014 |
| Last update date |
May 02, 2017 |
| Contact name |
Neil A. Mabbott |
| E-mail(s) |
neil.mabbott@roslin.ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
The Roslin Institute & Royal (Dick) School of Veterinary Sciences
|
| Street address |
Easter Bush
|
| City |
Midlothian |
| ZIP/Postal code |
EH25 9RG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE55722 |
Effect of lymphotoxin beta receptor blockade on microRNA expression in the spleen |
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| Relations |
| BioProject |
PRJNA240705 |
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