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| Status |
Public on Mar 01, 2014 |
| Title |
Tissue culture model of hypothermia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Therapeutic hypothermia is a clinically effective treatment for various hypoxic and ischemic conditions, but the associated molecular mechanisms remain unclear. To gain insight into hypothermia-induced transcriptional response, mouse embryonic fibroblasts were exposed to mild hypothermia (32°C) or normothermia (37°C) for increasing time periods. We aimed to identify genes with temporally near-monotonic response as the most obvious candidates for mediating the therapeutic effects of hypothermia.
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| Overall design |
Hypothermia was compared against normothermia at seven different time-points. Hypothermic and normothermic mouse embryonic fibroblasts (MEF, in 100 mm dishes, one array per time-point) were submitted to gene expression analysis using Mouse Gene 1.0 ST GeneChip® (Affymetrix) microarrays. Differential gene expression analysis was performed with R package DEMI (http://biit.cs.ut.ee/demi/).
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| Contributor(s) |
Ilmjärv S, Luuk H, Hundhal CA, Reimets R, Niitsoo M, Kolde R, Vilo J, Vasar E |
| Citation(s) |
24586062 |
| Submission date |
Jan 21, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Sten Ilmjärv |
| E-mail(s) |
sten.ilmjarv@gmail.com
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| Organization name |
University of Tartu
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| Department |
Institute of Biomedicine and Translational Medicine
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| Lab |
Department of Physiology
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| Street address |
Ravila 19
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| City |
Tartu |
| State/province |
Tartumaa |
| ZIP/Postal code |
50411 |
| Country |
Estonia |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA235997 |
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