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| Status |
Public on Dec 07, 2013 |
| Title |
Gene expression profilling of anaplastic thyroid carcinomas (ATC) and normal thyroid tissues |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
ATC are among the most lethal malignancies, for which there is no effective treatment.
ATC global gene expression was profiled and compared to normal thyroid tissues, in order to elucidate the molecular alterations contributing to ATC development, and to identify novel therapeutic targets.
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| Overall design |
RNA were extracted from 5 ATC samples. 3 samples were obtained at time of surgery, and were immediately frozen in liquid nitrogen. The RNA from the remaining samples were collected during fine-needle aspiration biopsies, being conserved in RLT buffer (RNeasy Mini Kit, Quiagen) and maintained at -70ºC. RNA were also extracted from 3 normal thyroid samples, which were collected during surgery, taken from the opposite lobe of thyroid tumors. We also hybridized a commercial pool of human thyroid total RNA (BD Bioscience).
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| Contributor(s) |
Pita JM, Leite V, Cavaco BM |
| Citation(s) |
24423316 |
| Submission date |
Dec 06, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Branca Maria Cavaco |
| E-mail(s) |
bcavaco@ipolisboa.min-saude.pt
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| Organization name |
Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG)
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| Department |
Unidade de Investigação de Patobiologia Molecular (UIPM)
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| Street address |
Rua Prof. Lima Basto
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| City |
Lisboa |
| ZIP/Postal code |
1099-023 |
| Country |
Portugal |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM1281639 |
Normal thyroid, fresh-frozen, biological rep2 |
| GSM1281640 |
Normal thyroid, fresh-frozen, biological rep3 |
| GSM1281641 |
ATC, fresh-frozen, biological rep3 |
| GSM1281642 |
ATC, fine-needle aspiration biopsy, biological rep4 |
| GSM1281643 |
ATC, fine-needle aspiration biopsy, biological rep5 |
| GSM1281644 |
Normal thyroid, commercial RNA pool |
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| Relations |
| BioProject |
PRJNA230799 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53072_RAW.tar |
39.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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