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| Status |
Public on Jan 01, 2014 |
| Title |
Eye-Specific Gene Expression following Embryonic Ethanol Exposure in Zebrafish: Roles for Heat Shock Factor 1 |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
Ethanol is a well-known teratogen. While this teratogenic potential is well-characterized clinically, the mechanisms through which ethanol exposure results in developmental defects remain unclear. Here we use the zebrafish model to elucidate eye-specific mechanisms that may underlie ethanol-mediated microphthalmia (reduced eye size), using time-series microarray analysis of gene expression of eye tissues of embryos exposed to 1.5% ethanol vs. untreated embryos. We identified 62 genes differentially expressed in ethanol-treated as compared to control zebrafish eyes from all sampling times over the period of retinal neurogenesis (24-48 hours post-fertilization). Application of the EDGE (extraction of differential gene expression) algorithm identified over 3000 genes differentially expressed over developmental time in ethanol-treated embryo eyes as compared to untreated embryo eyes. These lists included several genes indicating a mis-regulated cellular stress response (heat shock response) due to ethanol exposure. Combined treatment with sub-threshold levels of ethanol and a morpholino (MO) targeting heat shock factor 1 (hsf-1) mRNA resulted in a microphthalmic phenotype, suggesting convergent molecular pathways. Manipulation of the heat shock response by thermal preconditioning partially prevented ethanol-mediated microphthalmia while maintaining Hsf-1 expression. Together these data are consistent with roles for reduced Hsf-1 in mediating microphthalmic effects of embryonic ethanol exposure in zebrafish.
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| |
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| Overall design |
time series, 9 samples, no replicates
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| |
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| Contributor(s) |
Stenkamp D, Kashyap B |
| Citation(s) |
24355176 |
| Submission date |
Oct 18, 2013 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Deborah Lynn Stenkamp |
| E-mail(s) |
dstenkam@uidaho.edu
|
| Phone |
208-885-8963
|
| Organization name |
University of Idaho
|
| Department |
Biological Sciences
|
| Street address |
875 Perimeter Drive, MS 3051
|
| City |
Moscow |
| State/province |
Idaho |
| ZIP/Postal code |
83844-3051 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA223154 |
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