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| Status |
Public on Oct 25, 2013 |
| Title |
Gene Expression in wild type and CCR5 knockout mice with lung metastasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection.
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| Overall design |
There were 6 groups or conditions (CCR5 - 6 hours, WT - 6 hours, CCR5 - 24 hours, WT - 24 hours, CCR5 - 48 hours, WT - 48 hour); each group had 4 to 5 mice. All of the mice were injected with 100,000 B16 F10 melanoma cells by tail vein. At the designated time, the mice were anesthetized with Avertin and their lungs were perfused with PBS. After this, the lungs were harvested and snap frozen in liquid nitrogen. They were kept at minus 80 degrees C until processing. mRNA was extracted as described below and equivalent amounts of mRNA (by weight) was pooled by group.
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| Contributor(s) |
van Deventer HW, Serody JS, P-Y Ting J |
| Citation(s) |
24197118 |
| Submission date |
Oct 18, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Hendrik Wilhelm van Deventer |
| E-mail(s) |
hvand@med.unc.edu
|
| Organization name |
University of North Carolina Chapel Hill
|
| Department |
Medicine
|
| Street address |
170 Manning Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM1245191 |
CCR5 Lung 6 hrs post B16 F10 injection Replicate 1 |
| GSM1245193 |
CCR5 Lung 24 hrs post B16 F10 injection Replicate 1 |
| GSM1245194 |
CCR5 Lung 48 hrs post B16 F10 injection Replicate 1 |
| GSM1245196 |
WT Lung 6 hrs post B16 F10 injection Replicate 1 |
| GSM1245198 |
WT Lung 24 hrs post B16 F10 injection Replicate 1 |
| GSM1245200 |
WT Lung 48 hrs post B16 F10 injection Replicate 1 |
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| Relations |
| BioProject |
PRJNA224557 |
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