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Series GSE51392 Query DataSets for GSE51392
Status Public on Oct 18, 2013
Title Expression data from airway epithelial cells stimulated with Poly(I:C) from patients with asthma, rhinitis, and healthy controls
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Rhinovirus infections are the most common cause of asthma exacerbations. The complex responses by the airway epithelium to rhinovirus can be captured by gene expression profiling. We hypothesized that the upper and lower airway epithelium exhibit differential responses to double-stranded RNA (dsRNA), and that this is modulated by the presence of asthma and allergic rhinitis.
Identification of dsRNA-induced gene expression profiles by microarray of primary nasal and bronchial epithelial cells from the same individuals and examining the impact of allergic rhinitis with and without concomitant allergic asthma on expression profiles.
 
Overall design 17 subjects were included in a cross-sectional study (6 allergic asthma and allergic rhinitis; 5 allergic rhinitis; 6 healthy controls). RNA was extracted from isolated and cultured epithelial cells that were stimulated with Poly(I:C) for 24 hours from bronchial brushes and nasal biopsies, and analyzed by microarray (Affymetrix U133+ PM Genechip Array).
 
Contributor(s) Wagener AH, Luiten S, Zwinderman AH, van Drunen CM, Sterk PJ
Citation(s) 24475887
Submission date Oct 18, 2013
Last update date Dec 17, 2020
Contact name Ariane Wagener
E-mail(s) A.H.Wagener@amc.uva.nl
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1015 AZ
Country Netherlands
 
Platforms (1)
GPL13158 [HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate
Samples (68)
GSM1244586 baseline_bronchial_epithelium_rhinitis_subject (10_B05)
GSM1244587 polyI:C_bronchial_epithelium_rhinitis_subject (12_D07)
GSM1244588 baseline_nasal_epithelium_healthy_subject (13_F09)
Relations
BioProject PRJNA222885

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Supplementary file Size Download File type/resource
GSE51392_RAW.tar 142.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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