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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 19, 2013 |
| Title |
Essential functions for ID proteins at multiple checkpoints in natural killer T cell development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Thymic iNKT cell development is divided into four stages (stage 0-3) that are characterised, in C57BL/6 mouse strain, by the differential expression of surface markers, such as CD24, CD44 and NK1.1. During transition from immature to mature iNKT cell subsets, gene expression is tightly regulated. Here, we used microarray analysis to detail the influence of the transcriptional regulator ID3 during iNKT cell maturation in the thymus.
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| Overall design |
Stage 1 (Tet+CD24-CD44-NK1.1-) and Stage 2 (Tet+CD24-CD44+NK1.1-) iNKT cells from WT and Id3KO thymi were FACS sorted for RNA extraction and Affymetrix hybridisation.
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| Contributor(s) |
Kee BL, Sigvardsson M |
| Citation(s) |
24244015 |
| Submission date |
Sep 17, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Barbara L Kee |
| Organization name |
The University of Chicago
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| Department |
Pathology
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| Street address |
924 E57th st
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| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA219391 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50933_RAW.tar |
31.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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