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| Status |
Public on Feb 26, 2014 |
| Title |
DNA methylome profiling of human tissues identifies global and tissue-specific methylation patterns |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Background:
DNA epigenetic modifications, such as methylation, are important regulators of tissue differentiation, contributing to processes of both development and cancer. Profiling the tissue-specific DNA methylome patterns will provide novel insights into normal and pathogenic mechanisms, as well as help in future epigenetic therapies. In this study, 17 somatic tissues from four autopsied humans were subjected to functional genome analysis using the Illumina Infinium HumanMethylation450 BeadChip, covering 486 428 CpG sites.
Results:
Only 2% of the CpGs analyzed are hypermethylated in all 17 tissue specimens; these permanently methylated CpG sites are located predominantly in gene-body regions. In contrast, 15% of the CpGs are hypomethylated in all specimens and are primarily located in regions proximal to transcription start sites. A vast number of tissue-specific differentially methylated regions are identified and considered likely mediators of tissue-specific gene regulatory mechanisms since the hypomethylated regions are closely related to known functions of the corresponding tissue. Finally, a clear inverse correlation is observed between promoter methylation within CpG islands and gene expression data obtained from publicly available databases.
Conclusions:
This genome-wide methylation profiling study identified tissue-specific differentially methylated regions in 17 human somatic tissues. Many of the genes corresponding to these differentially methylated regions contribute to tissue-specific functions. Future studies may use these data as a reference to identify markers of perturbed differentiation and disease-related pathogenic mechanisms.
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| Overall design |
DNA methylation analysis of the total 72 tissue samples and controls was performed with the Illumina Infinium HumanMethylation450 BeadChip. The 17 post-mortem human somatic tissues used in this study were collected at the time of autopsy. Controls for unmethylated and methylated DNA were represented, respectively, by whole-genome amplified DNA from subcutaneous adipose tissue (using the GenomiPhi DNA amplification kit; GE Healthcare, Piscataway, NJ, USA) and the universal methylated human DNA standard (Zymo Research). We had two technical and two biological replicates processed by chip technique.
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| Contributor(s) |
Lokk K, Modhukur V, Rajashekar B, Märtens K, Koltšina M, Mägi R, Kolde R, Nilsson TK, Vilo J, Salumets A, Tõnisson N |
| Citation(s) |
24690455, 27004446 |
| Submission date |
Aug 26, 2013 |
| Last update date |
Oct 02, 2019 |
| Contact name |
Kaie Lokk |
| E-mail(s) |
kaie.lokk@ut.ee
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| Organization name |
University of Tartu
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| Street address |
Riia 23
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| City |
Tartu |
| ZIP/Postal code |
501010 |
| Country |
Estonia |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (70)
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| Relations |
| BioProject |
PRJNA217218 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50192_GPL13534_Matrix_raw_data.txt.gz |
138.0 Mb |
(ftp)(http) |
TXT |
| GSE50192_GPL13534_Matrix_signal_intensities_raw_data.txt.gz |
153.9 Mb |
(ftp)(http) |
TXT |
| GSE50192_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
| Processed data are available on Series record |
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