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| Status |
Public on Mar 11, 2014 |
| Title |
Comparison of RNA-Seq and Microarray in Transcriptome Profiling During T Cell Activation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Samples in this study probe the gene expression kinetics in human CCR6+ Th17 memory T cells activated under Th17 condition. Human CCR6+ Th17 memory T cells were purified from PBMC and gene expression was studied over a time course of 3 days after activation under Th17 condition. RNA from these samples was also profiled using RNA-Seq to compare different transcriptome profiling technologies.
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| Overall design |
PBMCs were purified from whole blood from a healthy donor. CD4+ cells were isolated by negative selection, followed by CCR6+ purification by positive selection. Two replicates of 6 time points were taken following stimulation in Th17 polarizing conditions.
This submission represents microarray component of study.
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| |
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| Contributor(s) |
Zhao S, Fung-Leung W, Liu X, Bittner A, Fan H, Ngo K, Stephens S |
| Citation(s) |
24454679 |
| Submission date |
Jul 17, 2013 |
| Last update date |
Apr 20, 2018 |
| Contact name |
Anton Bittner |
| E-mail(s) |
abittner@its.jnj.com
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| Organization name |
Janssen R&D
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| Department |
Systems Biology
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| Street address |
3210 Merryfield Row
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA212514 |
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