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| Status |
Public on Aug 08, 2013 |
| Title |
Human liver biopsy of different phases from control to NASH |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder in industrialized countries. Liver samples from morbidly obese patients (N=45) with all stages of NAFLD and controls (N=18) were analysed by array-based DNA methylation and mRNA expression profiling. NAFLD-specific expression and methylation differences were seen for nine genes coding for key enzymes in intermediate metabolism (including PC, ACLY, PLCG1) and insulin/insulin-like signalling (including IGF1, IGFBP2, PRKCE) and replicated by bisulfite pyrosequening (independent N=39). Transcription factor binding sites at NAFLD-specific CpG sites were >1000-fold enriched for ZNF274, PGC1A and SREBP2. Intra-individual comparison of liver biopsies before and after bariatric surgery showed NAFLD-associated methylation changes to be partially reversible. Post-bariatric and NAFLD-specific methylation signatures were clearly distinct both in gene-ontology and transcription factor binding site analyses, with >400-fold enrichment of NRF1, HSF1 and ESRRA sites. Our findings provide one of the first examples of treatment-induced epigenetic organ remodelling in humans.
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| Overall design |
73 samples of human liver grouped into C (control=14), H (healthy obese=27), S (steatosis=14) and N (nash=18).
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| Contributor(s) |
Jochen H, Andreas T, Timo I |
| Citation(s) |
23931760 |
| Submission date |
Jul 01, 2013 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Timo Itzel |
| E-mail(s) |
Timo.Itzel@klinik.uni-regensburg.de
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| Organization name |
Universitätsklinikum Regensburg
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| Street address |
Franz-Josef-StrauĂ-Allee 11
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| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (73)
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| GSM1178973 |
liver, C, A1359-04 |
| GSM1178974 |
liver, C, A1359-05 |
| GSM1178975 |
liver, N, A1359-06 |
| GSM1178976 |
liver, H, A1359-07 |
| GSM1178977 |
liver, C, A1359-08 |
| GSM1178978 |
liver, C, A1359-09 |
| GSM1178979 |
liver, C, A1359-10 |
| GSM1178980 |
liver, N, A1359-11 |
| GSM1178981 |
liver, H, A1359-12 |
| GSM1178982 |
liver, H, A1359-13 |
| GSM1178983 |
liver, H, A1359-14 |
| GSM1178984 |
liver, H, A1359-15 |
| GSM1178985 |
liver, H, A1359-16 |
| GSM1178986 |
liver, S, A1359-17 |
| GSM1178987 |
liver, N, A1359-18 |
| GSM1178988 |
liver, S, A1359-19 |
| GSM1178989 |
liver, S, A1359-20 |
| GSM1178990 |
liver, H, A1359-22 |
| GSM1178991 |
liver, H, A1359-23 |
| GSM1178992 |
liver, H, A1359-24 |
| GSM1178993 |
liver, S, A1649-01 |
| GSM1178994 |
liver, H, A1359-25 |
| GSM1178995 |
liver, N, A1359-26 |
| GSM1178996 |
liver, N, A1359-27 |
| GSM1178997 |
liver, H, A1359-28 |
| GSM1178998 |
liver, C, A1359-29 |
| GSM1178999 |
liver, S, A1359-30 |
| GSM1179000 |
liver, H, A1359-31 |
| GSM1179001 |
liver, N, A1359-32 |
| GSM1179002 |
liver, N, A1359-33 |
| GSM1179003 |
liver, N, A1359-34 |
| GSM1179004 |
liver, N, A1359-35 |
| GSM1179005 |
liver, H, A1359-36 |
| GSM1179006 |
liver, N, A1359-37 |
| GSM1179007 |
liver, H, A1359-38 |
| GSM1179008 |
liver, N, A1359-39 |
| GSM1179009 |
liver, C, A1649-02 |
| GSM1179010 |
liver, C, A1359-40 |
| GSM1179011 |
liver, S, A1649-03 |
| GSM1179012 |
liver, H, A1359-41 |
| GSM1179013 |
liver, H, A1359-42 |
| GSM1179014 |
liver, H, A1359-43 |
| GSM1179015 |
liver, N, A1359-44 |
| GSM1179016 |
liver, H, A1359-45 |
| GSM1179017 |
liver, N, A1359-46 |
| GSM1179018 |
liver, C, A1359-49 |
| GSM1179019 |
liver, H, A1359-50 |
| GSM1179020 |
liver, H, A1359-51 |
| GSM1179021 |
liver, S, A1649-05 |
| GSM1179022 |
liver, H, A1359-52 |
| GSM1179023 |
liver, S, A1359-53 |
| GSM1179024 |
liver, C, A1359-54 |
| GSM1179025 |
liver, S, A1649-08 |
| GSM1179026 |
liver, N, A1359-55 |
| GSM1179027 |
liver, S, A1649-06 |
| GSM1179028 |
liver, H, A1649-09 |
| GSM1179029 |
liver, S, A1359-56 |
| GSM1179030 |
liver, H, A1359-57 |
| GSM1179031 |
liver, C, A1649-10 |
| GSM1179032 |
liver, H, A1359-58 |
| GSM1179033 |
liver, N, A1359-59 |
| GSM1179034 |
liver, S, A1359-60 |
| GSM1179035 |
liver, N, A1359-61 |
| GSM1179036 |
liver, N, A1359-62 |
| GSM1179037 |
liver, S, A1359-63 |
| GSM1179038 |
liver, H, A1359-64 |
| GSM1179039 |
liver, N, A1649-11 |
| GSM1179040 |
liver, S, A1649-12 |
| GSM1179041 |
liver, H, A1649-13 |
| GSM1179042 |
liver, H, A1649-14 |
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| Relations |
| BioProject |
PRJNA210194 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48452_RAW.tar |
318.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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