 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 21, 2013 |
| Title |
Effect of chlorination on toxicity of wastewater effluents from different treatment systems to HepG2 human hepatoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Effect of chlorination on the toxicity of wastewater effluents treated by activated sludge (AS) and submerged membrane bioreactor (S-MBRB) systems to HepG2 human hepatoblastoma cells was investigated. In addition to cytotoxicity assay, the DNA microarray-based transcriptome analysis was performed to evaluate the change in modes of toxic actions (MOAs) of effluents by chlorination. Effluent organic matters (EfOM) and disinfection by-products (DBPs) were characterized by using Fourier transform mass spectrometry (FT-MS). The cytotoxicity of AS effluent was elevated by chlorination, while the toxicity of S-MBRB effluent was reduced. The averaged O/C ratio of EfOM in S-MBRB effluent was lower than that in AS effluent. The results of the transcriptome and FT-MS analyses suggested that lower O/C molecules influenced on “response to hormone stimulus” and “acute inflammatory response” but those were decreased by chlorination, which consequently reduced cytotoxicity. On the other hand, larger number of DBPs and other molecules were increased in AS effluents by chlorination. Those molecules might influence on “cellular metabolic process”, which consequently elevated cytotoxicity. Therefore, the combination of the toxicity assays and chemical analysis demonstrated the changes in severity of cytotoxicity and MOAs by chlorination, and the difference of chemical characteristics which relate to those toxicity changes.
|
| |
|
| Overall design |
We examined the gene expression alteration in human hepatoma cell line, HepG2 exposed to the chlorinated wastewater effluents from membrane bioreactor and the activated sludge process. Human Genome Focus Array, which represents 8,795 verified human sequences, was used. All effluent samples were concentrated by using solid phase extraction (SPE). SPE fraction from MQ water was used as controll. For duplicate, two dishes were prepared for each sample and individually treated in parallel.
|
| |
|
| Contributor(s) |
Fukushima T, Okabe S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 20, 2013 |
| Last update date |
Jul 08, 2016 |
| Contact name |
Toshikazu Fukushima |
| Organization name |
Hokkaido University
|
| Street address |
Kita13, Nishi8, Kita-ku
|
| City |
Sapporo |
| ZIP/Postal code |
060-8628 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL201 |
[HG-Focus] Affymetrix Human HG-Focus Target Array |
|
| Samples (16)
|
| GSM1171499 |
HepG2 S-MBR B 0mg/L Apr 12, 2012 rep2 |
| GSM1171500 |
HepG2 S-MBR B 2mg/L Apr 12, 2012 rep1 |
| GSM1171501 |
HepG2 S-MBR B 2mg/L Apr 12, 2012 rep2 |
| GSM1171502 |
HepG2 S-MBR B 5mg/L Apr 12, 2012 rep1 |
| GSM1171503 |
HepG2 S-MBR B 5mg/L Apr 12, 2012 rep2 |
| GSM1171504 |
HepG2 control Jun 12, 2012 rep1 |
| GSM1171505 |
HepG2 control Jun 12, 2012 rep2 |
| GSM1171506 |
HepG2 AS 0mg/L Jun 12, 2012 rep1 |
| GSM1171507 |
HepG2 AS 0mg/L Jun 12, 2012 rep2 |
| GSM1171508 |
HepG2 AS 2mg/L Jun 12, 2012 rep1 |
| GSM1171509 |
HepG2 AS 2mg/L Jun 12, 2012 rep2 |
| GSM1171510 |
HepG2 AS 5mg/L Jun 12, 2012 rep1 |
| GSM1171511 |
HepG2 AS 5mg/L Jun 12, 2012 rep2 |
|
| Relations |
| BioProject |
PRJNA209055 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48157_RAW.tar |
22.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...